Methods in Molecular Biology: Methods and Protocols

Abdul-Sater, Ali A.

This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, The Inflammasome: Methods and Protocols aims to provide protocols that will serve as tools for current and aspiring researchers interested in studying inflammasomes.

Details

Published by: Humana

Publication Date: 2023-02-27

Format: Paperback

ISBN-13: 9781071621462

DOI: 10.1007/978-1-0716-2144-8

Dimensions: 254cm x178cm

Pages: 180