Affinity Chromatography: Methods and Protocols

Ayyar, B. Vijayalakshmi; Arora, Sushrut

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Details

Published by: Humana

Publication Date: 2023-05-20

Format: Paperback

ISBN-13: 9781071621783

DOI: 10.1007/978-1-0716-2176-9

Dimensions: 254.0cm x178.0cm

Pages: 278.0