Poly(ADP-Ribose) Polymerase: Methods and Protocols

Tulin, Alexei V.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Details

Published by: Humana

Publication Date: 2022-12-15

Format: Hardcover

ISBN-13: 9781071628904

DOI: 10.1007/978-1-0716-2891-1

Dimensions: 254.0cm x178.0cm

Pages: 446.0