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Genetically Incorporated Non-Canonical Amino Acids
  • Genetically Incorporated Non-Canonical Amino Acids
Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols

Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols

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Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols

Tsai, Yu-Hsuan; Elsässer, Simon J.

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Details

Published by: Humana

Publication Date: 2023-06-06

Format: Hardcover

ISBN-13: 9781071632505

DOI: 10.1007/978-1-0716-3251-2

Dimensions: 254.0cm x178.0cm

Pages: 286.0

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