Methods in Molecular Biology: Methods and Protocols

Kawasaki, Tsutomu; Yoshioka, Hirofumi

This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.

Details

Published by: Humana

Publication Date: 2026-02-06

Format: Hardcover

ISBN-13: 9781071651377

DOI: 10.1007/978-1-0716-5138-4

Dimensions: 254cm x178cm

Pages: 266