This detailed new edition presents the fundamental principles and emerging technologies underlying freezing and drying of biospecimens through a variety of practical protocols. After sections exploring mathematical modeling, new technologies, and the importance of standard operating procedures in biorepositories and biobanks, the book continues with cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications, including for agricultural purposes, model organisms and biodiversity conservation, reproductive and regenerative medicine, and biologics for foods and pharmaceutics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Cryopreservation and Freeze-Drying Protocols, Fifth Edition serves as an ideal resource for researchers and technical staff in academia and industry who are interested in studying the fundamentals underlying freezing and drying or in establishing practical methods for the safe storage of biological specimens.