This volume details step-by-step protocols and troubleshooting tips to image immune cell behavior in real time, both in vitro and in vivo. Chapters describe complex experimental setups to monitor cell biophysics, as well as tools for cell signaling, phagocytosis, metabolism, chemokine responses, intravital imaging protocols covering a range of anatomical sites, and advanced analysis approaches for immune cell microscope datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Real-Time Imaging of Immune Cells: Methods and Protocols aims to be a comprehensive and accessible resource for researchers seeking to apply live imaging to the study of the immune system in order to facilitate the dissemination and continued development of these powerful methodologies.