This volume presents readers with a collection of detailed protocols for the detection, quantification, and functional analysis of H₂S and related sulfur species in biological systems. The chapters in this book cover a broad range of approaches such as accessible colorimetric and fluorometric assays, fluorescence microscopy, flow cytometry, extracellular flux analysis, chemoselective derivatization, and liquid chromatography–tandem mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Hydrogen Sulfide Signaling: Methods and Protocols is a valuable resource for investigators studying sulfur metabolism, sulfide bioavailability, enzymatic H₂S generation, donor release kinetics, mitochondrial responses, and protein persulfidation.