Methods in Molecular Biology: Methods and Protocols

Dumont, Marc G.; Hernández García, Marcela

This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Details

Published by: Humana

Publication Date: 2020-08-13

Format: Paperback

ISBN-13: 9781493997237

DOI: 10.1007/978-1-4939-9721-3

Dimensions: 254cm x178cm

Pages: 247