Methods in Molecular Biology: Methods and Protocols

Engelborghs, Yves; Visser, Antonie J.W.G.

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

Details

Published by: Humana

Publication Date: 2013-10-10

Format: Hardcover

ISBN-13: 9781627036481

DOI: 10.1007/978-1-62703-649-8

Dimensions: 254cm x178cm

Pages: 816