This book constitutes the refereed proceedings of the International Challenge on Medical Image Registration, Learn2Reg 2025, held in conjunction with MICCAI 2025, which took place in Daejeon, South Korea, during September 27–October 4, 2025.

The 11 full papers presented in the proceedings were carefully selected and reviewed from 38 submissions.  The papers cover two principle sub-tasks: ReMIND2Reg and LUMIR.