This book comprehensively reviews biomacromolecular crystallization across experimentation and modelling and, together with its companion volume "Fundamentals of Biomacromolecular Crystallization: Insights into Thermodynamics and Kinetics", offers a quantitative-oriented perspective, from thermodynamics and kinetics fundamentals to scalable continuous processes. Divided into 3 sections, the book begins with an overview of reported crystallizers for biomolecular crystallization experimentation, followed by a discussion about the use of process analytical technology (PAT) for the development of biomolecular crystallization. In the 3rd part of the book, readers will learn about the modelling of biomolecular crystallization processes. Expert contributors cover topics such as biomolecular crystallization in microfluidic devices, continuous crystallization platforms, in-situ online spectroscopy and light scattering techniques, and mathematical modelling applied for biomolecular crystallization. Particular attention is given to the recent developments in biomolecular crystallization modelling, including advancements in AI and ML.

This book serves as a valuable resource for designing and performing successful biomolecular crystallization experiments. Given its breadth, it appeals to scholars and researchers from both academia and industry, working in the field of biomolecular crystallization across several study areas, such as biochemistry, engineering, and biotechnology, among others.

Joana Ferreira is a Postdoctoral Researcher in the Department of Chemical Engineering at Massachusetts Institute of Technology, USA. She holds a dual PhD from KU Leuven and the University of Porto, specializing in protein crystallization, microfluidics, and protein solution behavior. Her research interests focus on biomacromolecules, crystallization and precipitation, pharmaceutical formulations, and multiphase microfluidics. Her work has appeared in leading journals such as Crystal Growth & Design and CrystEngComm, and she also contributed to Springer Nature’s Methods in Molecular Biology series, authoring a chapter on high‑throughput protein crystallization.

Filipa Castro is an Assistant Researcher at the Centre of Biological Engineering, at the University of Minho, Portugal. She holds a PhD in Bioengineering from the University of Minho, Portugal, and since 2009, her work has advanced from nanoparticle production and multiphase bioreactors to pioneering micro‑ and meso‑reactor technologies for protein crystallization. Her recent research focuses on continuous and sustainable crystallization methods for the selective purification of biopharmaceuticals. She is actively involved in major international projects, including BioNext, CrystalWhey, and PROCRYSTAL, contributing innovative strategies for efficient, scalable, and environmentally responsible bioprocessing.