This book highlights super-resolution imaging using Stochastic Optical Reconstruction Microscopy (STORM). It begins with describing an optical microscope and its resolution limit due to the diffraction phenomenon. It also describes how diffraction barriers can be overcome by various optical super-resolution techniques. The book then discusses how super-resolution imaging can be achieved with the help of a simple experimental setup using the stochastic nature of fluorescence emission. It further elaborates the requirements in terms of excitation beam, fluorophore types, the imaging buffer and the sample preparation methods to observe fluorescence blinking, followed by a discussion on the image acquisition and the reconstruction algorithms. The book concludes with applications of STORM, its challenges and limitations.

Dr. Bosanta Ranjan Boruah obtained his Ph.D. from the Photonics Group at Imperial College London, UK. He started his academic career by the joining the Guwahati University as a lecturer of Physics in the year 2001. He then moved IIT Guwahati in the year 2009. Presently, he is working as a professor at the department of Physics in IIT Guwahati. His research interests include programmable diffractive optics, optical microscopy, wavefront sensing, high numerical aperture focusing of light beams, optical trapping, and free space communication.

Anupam Bharadwaj obtained his M.Sc. degree in Physics from Guwahati University, India. He is presently a doctoral researcher in the Department of Physics, Indian Institute of Technology Guwahati, India, dedicated to the field of optical super-resolution microscopy using Stochastic Optical Reconstruction Microscopy, aka STORM. During his Ph.D., in the year 2022, he worked as a research assistant in the Department of Physics, Imperial College London, UK, where he was involved in building an open-source super-resolved optical microscope utilizing the easy STORM approach and worked with a modular imaging platform to develop a hyperspectral fluorescence imaging microscope. Anupam has developed skills and expertise in the instrumentation of affordable modular optical imaging platforms for widefield microscopy, scanning microscopy and sample preparation for superior localization microscopy implementing STORM, with a primary focus on applications in nanoparticles and biomedical imaging.

Pranjal Choudhury is a Ph.D. student in the Department of Physics at the Indian Institute of Technology Guwahati. With a keen interest in pushing the boundaries of optical imaging techniques, particularly in the field of super-resolution microscopy, he specializes in developing efficient image reconstruction modalities for single molecule localization microscopy (SMLM). Drawing from a strong background in physics, he focuses on advancing the capabilities of Stochastic Optical Reconstruction Microscopy (STORM) through innovative approaches to image processing and data analysis. He has demonstrated a commitment to bridging the gap between theory and experimentation, leveraging cutting-edge techniques to address complex challenges in microscopy.

Dr. Amalesh Kumar is a postdoctoral fellow specializing in super-resolved microscopic bioimaging, especially in Stochastic Optical Reconstruction Microscopy (STORM). He obtained his Ph.D. from the Indian Institute of Technology (Indian School of Mines) Dhanbad, India, with a background in fluorescence spectroscopy. He has developed nanophosphor materials for solid-state lighting and non-contact temperature sensing applications. He continues his postdoctoral position at the Department of Physics, Indian Institute of Technology Guwahati, collaborating with the Department of Biosciences and Bioengineering at the same Institute on super-resolved fluorescence imaging, especially in cancerous cell lines.